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脂解(脂肪細胞)檢測試劑盒
Lipolysis (Adipocyte) Kit
脂解(脂肪細胞)檢測試劑盒
Lipolysis (Adipocyte) Kit
基本信息
| EC number【EC 號】 | 259-322-7 |
| NACRES | NA.25 |
| General description【一般描述】 | Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes. |
| Principle【原理】 | The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present. |
| Components【組分】 | 組份不可單獨銷售 Collagenase (0.2%) Collagenase Stop Buffer Adipocyte Wash Buffer Adipocyte Lipolysis Buffer Glycerol Assay Buffer Glycerol Probe, in DMSO Glycerol Enzyme Mix Glycerol Standard, 100 mM Isoproterenol, 10 mM Cell Strainer |
產品性質
| Detection Method | colorimetric fluorometric |
| storage temp.【儲存溫度】 | −20℃ |
| suitability【適用性】 | 該試劑盒適用于從組織中分離脂肪細胞和脂解作用的測定。 |
安全信息
| Pictograms【象形圖】 | GHS07 |
| Signal word【警示用語：】 | Warning |
| Hazard Statements | H315 - H319 - H335 |
| Precautionary Statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Classifications【危險分類】 | Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【儲存分類代碼】 | 10 - Combustible liquids |
| WGK | WGK 3 |
